Table 2.

Primer sequences for multiplex PCR-RFLP

Mutation	Forward Primer	Reverse Primer	Product Size
G3460A	5'-CCCCTACGGGCTACTACAACCCTTCGCTGtC	5'-GATAGTAGAATGATGGCTAG	333 bp
G11778A	5'-AGCAAACTCAAACTACGAACG	5'-TTACTAGCACAGAGAGTTCTC	164 bp
T14484C	5'-AATAGCCATCGCTGTAGTATATCCAAAGACAgtCA	5'-GTGCGAGAATAATGATGTATGC	236 bp

Lowercase letters indicate alterations introduced to the forward primers, which when in combination with the mutation, generate a MaeIII site. PCR product sizes are indicated