Table 3.
PCR product and restriction product sizes expected in diagnostic test
| 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|
| Uncut PCR products | MaeIII Non-mutated | MaeIII 3460A | MaeIII 11778A | MaeIII 14484C |
| 333 | 307 | 279 | 307 | 307 |
| 236 | 236 | 236 | 236 | 205 |
| 164 | 164 | 164 | 135 | 164 |
| 28 | 29 | 31 | ||
| 26 | 26 | 26 | 26 |
Column 1: PCR product sizes (bp) generated in the multiplex PCR. Column 2: Size of MaeIII restriction products obtained from non-mutated DNA samples. Column 3: Size of MaeIII restriction products obtained from 3460A samples. Column 4: Size of MaeIII restriction products obtained from 11778A samples. Column 5: Size of MaeIII restriction products obtained from 14484C samples