Figure 7.

Transduction with Gfi1 retroviruses reestablishes the ILC2 phenotype and restricts the ILC3 phenotype in Bcl11b^−/− ILC2s. A) Flow cytometry analysis of the NGFR⁺ population in the Gfi1-NGFR- or NGFR-transduced lung Lin⁻CD90⁺Sca-1⁺CD127⁺ cells, isolated from TMX-Rag1^−/−Bcl11b^F/Fcre-ERT2 mice. B) Gata3, ST2, Rorγt, IL-23R and Rorα in NGFR⁺ Lin⁻CD90⁺Sca-1⁺CD127⁺ cells ((Gfi1-NGFR (black) and NGFR (dashed)). C) Average MFI of Gata3, ST2, Rorγt, IL-23R and Rorα in NGFR⁺ Lin⁻CD90⁺Sca-1⁺CD127⁺ cells (Gfi1-NGFR (black) or NGFR (white)). Data (n=4), derived from two independent experiments, are presented as mean ± SEM. Significance determined by Student's t test, * indicates p-value ≤ 0.05.