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. 2015 May 8;62(1):207–219. doi: 10.1002/hep.27694

Figure 5.

Figure 5

Effects of NTCP deficiency on the enterohepatic circulation. Bile was collected for 30 minutes after gallbladder cannulation and ligation of the common bile duct. (A) Bile flow (microliters per minute per 100 g body weight) is shown during this period for WT (open dots), normocholanemic Slc10a1−/− (black dots), and hypercholanemic Slc10a1−/− (black triangles) mice. (B) Bile acid output (nanomoles per minute per 100 g body weight) during the 10- to 20-minute collection period for WT (white bar), normocholanemic Slc10a1−/− (light gray bar), and hypercholanemic Slc10a1−/− (dark gray bar) mice. Data are given as mean ± SEM (n – 8-12). (C) Total fecal BA excretion (micromoles per 24 hours per kilogram of body weight) and (D) relative mRNA expression of BA transporters in the distal ileum for WT mice (white bars) and for Slc10a1−/− mice with low (light gray bars) and high (dark gray bars) serum BA concentrations, using the geometric mean of control genes Hprt and Ppib. (E) Urinary BA concentrations (μmol/L) and (F) relative mRNA expression of renal BA transporters for WT (white bars) and for Slc10a1−/− mice with low (light gray bars) and high (dark gray bars) serum BA concentrations, using the geometric mean of control genes Rplp0 and Hprt. Data in (C-F) are given as mean ± SEM (n – 4-6) from 6- to 8-week-old mice. *P < 0.05 (compared to WT levels).