FIG. 4.

MnSOD depletion affects anchorage-independent and anchorage-dependent cell growth. (A) Anchorage-independent and anchorage-dependent growth of HepG2-sc and MnSOD-kd cells was assessed by colony formation and soft agar assay, respectively. Data are presented as sum of colony volume [Σ(V)] and sum of area [Σ(A)], respectively. (B) Representative images from soft agar and colony formation assay are shown. (C) Migration was determined by a transwell assay (8 μm pore size) under serum starvation. Data are mean ± SD of the fold induction normalized to HepG2-sc cells. Representative images and quantification of migrated cells are shown. (D) Cell adhesion was assessed by rotating cells at 250 rpm on an orbital shaker for 2 h at 37°C, while keeping a control plate motionless. The number of adherent cells in the motionless control plates was set to 100%; data are mean ± SD (*Significant difference, p < 0.05). (E) Representative images of crystal violet stained cells still attached to the plate are shown. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars