FIG. 5.

Hepatocyte-specific MnSOD KO in mice induces 8-isoprostane. (A) Immunoblot analyses for MnSOD in protein lysates prepared from liver, kidney, and brain of the MnSOD^flox/flox control mice (WT) or hepatocyte-specific MnSOD-KO mice. One hundred microgram of protein was subjected to Western blot analyses with antibodies against MnSOD and β-actin. The β-actin is shown for equal loading of proteins. (B) Scheme of the superoxide-mediated peroxidation of an arachidonic acid (Ara) containing lipid. (C) Liver sections from 3-month-old, male MnSOD^flox/flox control mice (WT) and MnSOD-KO mice (KO) were prepared and immunostained for 8-isoprostane. 400× magnification. (D) 8-Isoprostane staining normalized to WT and presented as mean ± SD (n = 6/group; *p < 0.05). KO, knockout; WT, wild type. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars