FIG. 3.

OSS induced oxidative stress and JNK activation to activate autophagy. (A) HAECs were infected with control or recombinant MnSOD adenovirus (MOI 1:100) overnight, and then were exposed to static condition or OSS for 4 h. Overexpression of MnSOD significantly attenuated OSS-induced LC3-II/LC3-I ratios (OSS: p<0.05, n=4; MnSOD: p<0.05, n=4). (B) HAECs were exposed to static condition or OSS for 4 h in the presence or absence of NAC at 5 mM or (C) a JNK inhibitor, SP600125 (SP), at 2 μM. Both antioxidant NAC and JNK inhibitor significantly attenuated OSS-induced LC3-II/LC3-I ratios (NAC: p<0.01; SP: p<0.05, n=4). JNK, c-Jun N2-terminal kinase; MnSOD, manganese superoxide dismutase; MOI, multiplicity of infection; NAC, N-acetyl cysteine.