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. Author manuscript; available in PMC: 2015 Nov 24.
Published in final edited form as: Cancer Biol Ther. 2008 Nov 12;7(11):1774–1782. doi: 10.4161/cbt.7.11.6781

Figure 5.

Figure 5

(A) Immunohistochemical analysis of MMP-2, -9, -3 and -11 in day 44 MCF10DCIS.com xenografts. Note that upregulated expression of proteases is associated with breach of basement membrane as revealed by type IV collagen staining (arrow in a). Original magnification, x10 (b–e); x40 (a). Panel (f), gelatin zymography of total cell lysates or conditioned media of passages 13, 28 and 46 MCF10DCIS.com cells. Panel (g), Western blot analysis of MMP-2 and -9 in cell lysate (LYS) and conditioned media (CM) of passage 46 MCF10DCIS.com cells. (B) Immunohistochemical analysis of MMP-2, -9, -3 and -11 in clinical comedo-DCIS breast tumors. Note the presence of intense immunoreactivity to the indicated MMP in the stromal and epithelial compartments of solid (duct 2) and comedo (duct 1) DCIS lesions. Note the presence of strong reactivity to MMP-2 antibody in a duct showing very early signs of comedo formation (d) and the continued overexpression in a comedo-DCIS lesion showing lymphocytic infiltration (e). Original magnification, x10 (a and b); x20 (c and d); x40 (e).