Skip to main content
. 2015 Sep 17;64(12):4158–4170. doi: 10.2337/db15-0239

Figure 6.

Figure 6

ChREBPβ depletion attenuates glucose-stimulated β-cell proliferation in INS-1–derived 832/13 cells. A: INS-1–derived 832/13 cells were treated with control siRNAs (siCon) or siRNAs targeted against ChREBPβ mRNA (Siβ-01 and -02) and 24 h later cultured for 16 h in 2 or 20 mmol/L glucose. Immunoblotting was performed using antibodies against ChREBP (ChREBPα is visualized) and β-actin (note that these siRNAs decreased ChREBPβ mRNA but had no effect on ChREBPα, Fig. 1). pos, positive control. B: After 48 h of the siRNA treatment, cells were treated for 2 h with 2 or 20 mmol/L glucose and fixed and stained with an antibody recognizing ChREBPα. C: After 48 h, cells were cultured in 2 or 20 mmol/L glucose for 16 h, and BrdU was added 30 min before fixation and staining for BrdU (red) and DAPI (blue). D: Quantification of the results in C, wherein at least 1,000 cells were counted. Results are from four independent experiments. Error bars are the SEM. siChREBPβ, siRNAs directed against ChREBPβ. *P < 0.05.