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. 2015 Sep 17;64(12):4158–4170. doi: 10.2337/db15-0239

Figure 7.

Figure 7

Depletion of ChREBPβ attenuates glucose-stimulated β-cell proliferation in isolated rat islet cells. A and B: Isolated rat islet cells were treated with lipid-conjugated (Accell) siRNAs and cultured in 5.5 or 15 mmol/L glucose for 96 h. Total RNA was collected and subjected to RT-PCR using primers specific for ChREBPβ or ChREBPα mRNAs. C: Protein extracts were subjected to immunoblotting using an antibody against ChREBPα and β-actin. D: To determine the effects of the siRNAs on ChREBP translocation, cells were treated with control or Accell siRNA against ChREBPβ cultured in 5.5 or 15 mmol/L glucose for 32 h and were fixed and stained for insulin, ChREBP, and DNA (DAPI). E: Isolated rat islet cells were treated with Accell siRNAs and cultured in 5.5 or 15 mmol/L glucose for 96 h and fixed and stained with Ki67 and insulin. F: Quantification of the results in E, wherein at least 1,000 insulin-positive cells were counted. Results are from four different rat islet isolations. siChREBPβ-01 and -02, siRNAs directed against ChREBPβ; siCon, scramble control siRNA. Error bars are the SEM. *P < 0.05.