Figure 2.

P2Y₁₂-R expression in rat kidney. (A) P2Y₁₂-R mRNA expression relative to that of β-actin in the three major regions of the kidney and other organs of rat. CTX, cortex; OM, outer medulla; IM, inner medulla. Bars show mean±SEM of triplicate real-time PCR assays, plotted relative to the expression levels in the brain. (B) P2Y₁₂-R protein in the rat kidney and brain. Identical immunoblots prepared by applying equal amounts of protein (30 µg) from solubilized homogenates of rat brain (BR), renal cortex (CTX), or outer medulla (OM) or inner medulla (IM) were incubated with the affinity purified P2Y₁₂-R antibody (Ab.) or the antibody blocked by the immunizing peptide or with protein A-purified preimmune (Imm.) IgG as marked on the top of the panels.