Figure 3.

Immunolocalization of P2Y₁₂-R protein in rat kidney. Upper row: Immunoperoxidase labeling for P2Y₁₂-R: (A) labeling of brush border membrane of proximal tubules (PT); (B) peptide-block ablated the labeling in PT; (C) substitution of antibody with preimmune (Imm.) IgG showed no labeling in the PT (arrows); weak labeling on the apical aspect of the CD in the medulla (D), and cortex (E) (arrows). Middle and lower rows: IF labeling of AQP2 (green) and P2Y₁₂-R (red): (F) and (G) are low magnification profiles of cortical region, showing labeling of arterioles (arrowhead in F), proximal tubules (arrows in F), and cortical CDs showing merging of labeling of AQP2 and P2Y₁₂-R (arrows in G). (H) Low magnification profile of inner medullary region showing labeling of CDs for AQP2 and P2Y₁₂-R. (I)–(K) High magnification images of inner medullary region showing labeling of medullary CDs for AQP2 or P2Y₁₂-R or merging of both. Cell nuclei were labeled with DAPI. Bars are 20 µm in length.