Skip to main content
. 2015 Apr 8;26(12):2963–2977. doi: 10.1681/ASN.2014050528

Figure 5.

Figure 5.

TxNIP−/− mice do not demonstrate increased ROS/reactive nitrogen species and Nox4 expression in response to hyperglycemia. Evidence of ROS/reactive nitrogen species and Nox4 expression in nondiabetic and diabetic TxNIP WT and KO mice. (A) Representative images of glomerular reactive nitrogen species as indicated by nitrotyrosine staining and quantification (bottom panel) (n=5 mice/condition). (B) Urinary 8-OhdG levels (pg), reflective of ROS, in 24-hour urine collections from 6 to 7 mice/condition. Results are mean±SEM. **P<0.001 and ***P<0.0001 diabetic WT mice versus nondiabetic and diabetic TxNIP KO mice. (C) Representative images and quantitation of glomerular Nox4 protein in (n=5 mice/condition). (D) Expression of Nox4 mRNA, corrected for the housekeeping gene actb and normalized to nondiabetic WT in renal cortex. Results are mean±SEM (n=5 mice/condition). *P<0.05 diabetic WT mice versus nondiabetic WT and diabetic KO; **P<0.01 nondiabetic TxNIP KO mice versus nondiabetic WT; ***P<0.001 diabetic WT mice versus nondiabetic WT; ††P<0.001 nondiabetic KO versus nondiabetic WT, and #P<0.05; ##P<0.001; and ###P<0.0001 diabetic WT versus diabetic TxNIP KO mice.