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. Author manuscript; available in PMC: 2015 Nov 24.
Published in final edited form as: J Clin Cell Immunol. 2015 Jun 20;6(3):334. doi: 10.4172/2155-9899.1000334

Figure 1.

Figure 1

Microfluidic droplet generation and docking microarray platform. (A) PDMS nanoliter droplet array device showing inlets for oil (highlighted by arrows), T cells (inlet 1) and ionomycin or DCs (inlet 2). (B–D) Images of the flow of two input streams containing red and green fluorescent polystyrene beads (7 μm diameter) in the region indicated in (A). The aqueous and oil flow rates are 50 μl/hour and 300 μl/ hour. The fluorescent exposure was kept in the range of 500–700 msec, which precludes observation of individual beads in the serpentine region under flow. (E) Droplet generation at flow-focusing junction. Droplets containing T cells are indicated by arrowheads. (F) Generated droplets driven towards the docking microarray. (G) Droplet-filled microarray. (H) Droplets containing single T cells (indicated by arrowheads) labeled with Fluo-4. Scale bar: 50 μm.