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. 2015 Nov 23;10(11):e0143441. doi: 10.1371/journal.pone.0143441

Fig 3. Bcl-w and Survivin are direct downstream targets of miR-203.

Fig 3

(A) Illustration of the predicted miR-203 targeting sites in 3’UTR regions of Bcl-w and Survivin. (B) Dual-Luciferase activity assay was performed in HEK293T cells co-transfected with control/ miR-203 and pmiR-REPORT vectors with wild-type/mutant 3′-UTR of Bcl-w (above) and Survivin (below). *P<0.01, t test, n = 6. (C) Western blots showing downregulation of Bcl-w and Survivin proteins after transfection of miR-203 mimics in 5637 and T24 cell lines. β-actin was used as a control. (D) RT-qPCR showing downregulation of Bcl-w and Survivin mRNAs after transfection of miR-203 mimics in 5637 and T24 cell lines. *P<0.01, t test, n = 6. (E) Spearman’s correlation analysis showing a significant inverse association between miR-203 and Bcl-w mRNA or Survivin mRNA expression in bladder cancer tissues(r = -0.781, -0.740, both at P<0.001).