Fig 3. Map-based cloning of w67.

A, The w67 locus was mapped to short arm of chromosome 3 between SSR markers RM14288 and RM14412; B, The w67 locus was narrowed down to about 274-kb region between RM6013 and RM569 using 298 F₂ mutant individuals from cross of w67/R9308; C, The w67 locus was further mapped to about 160-kb region covering three BAC clone AP009053, AC098693 and AC140005 between InDel maker ID32 and RM569 using 503 F2 mutant type plants from cross of w67/Moroberekan; D, 26 ORFs were predicted in the mapped region and a single nucleotide substitution from A to T at position 130 in LOC_Os03g03990 was identified. Markers used for mapping are listed in S2 Table.