Table 3. Effects of inhibitors on the farnesol dehydrogenase activity.
The enzyme was preincubated for 5 min at 35°C with the various reagents before addition of the substrate. Each reagent was added at the final concentration as indicated.
| Reagent (1.0 mM) | Relative activity (%) |
|---|---|
| None | 100 |
| 2,2-Dipyridil | 48 |
| Iodoacetamide | 34 |
| Sodium azide | 75 |
| 5,5’-Dithiobis (2-nitrobenzoic acid) | 0 |
| p-Chloromercuribenzoate | 0 |
| EDTA | 0 |
| 1, 10-Phenanthroline | 0 |
| Lithium chloride | 0 |
| Silver nitrate | 0 |
| Zinc chloride | 0 |
| Magnesium chloride | 27 |
| Calcium chloride | 76 |
| Iron (III) chloride | 0 |
| Cuprum sulphate | 0 |