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. 2015 Nov 23;10(11):e0143467. doi: 10.1371/journal.pone.0143467

Fig 5. Knockdown of VPg and eIF4E protein interactions decreased the levels of RHDV translation.

Fig 5

RK13 cells were co-transfected with the p4EBP1 (0 μg, 0.3 μg, 0.6 μg, or 1.0 μg), pRHDV-luc (1 μg), peGFP (0.5μg) and pIRES-Rluc (0.5 μg) plasmids. (A) RK13 cells were lysed at 48 h post-transfection, and the levels of 4EBP1, eGFP and Rluc were determined by immunoblotting with antibodies against the indicated proteins. (B) RK13 cells were lysed at 48 h post-transfection, and Fluc activity was measured based on RLUs and normalized according to the results obtained for a co-transfected plasmid encoding Renilla luciferase. The experiments were conducted in duplicate, and similar results were obtained in two independent experiments.