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. 2015 Nov 23;10(11):e0143467. doi: 10.1371/journal.pone.0143467

Fig 6. Silencing of eIF4E inhibited RHDV translation.

Fig 6

(A) The expression of eIF4E was silenced by siRNA. The 293T cells transfected without siRNA (Mock), eIF4E siRNA (si-eIF4E), untreated (No treat), or treated with different concentrations (nM) of si-eIF4E (as indicated at the top of each lane) were harvested at 48 h post-transfection. The expression of eIF4E was measured by immunoblotting with antibodies against the indicated proteins. (B) The translation of RHDV in eIF4E-silenced siRNA, pRHDV-luc and peGFP plasmids co-transfected cells were conducted as described in panel A. The cells were lysed at 48 h post-transfection, and Fluc activity was measured based on RLUs and normalized according to the results obtained for a co-transfected pIRES-Rluc plasmid encoding Renilla luciferase. The experiments were conducted in duplicate, and similar results were obtained from two independent experiments. The Student’s t test and ANOVA were applied for the statistical analyses, P <0.01 was considered as extremely significant different (**).