Fig 8. Rotundifolone-induced Ca²⁺ transient in myocytes depend of extracellular Ca²⁺.

(A) Representative recording of the Ca²⁺ signals induced by rotundifolone (10⁻³ and 3x10^-3 M) in freshly dispersed vascular smooth muscle cells from rat superior mesenteric artery. (B) Summary data showing the amplitude of the Ca²⁺ signal induced after perfusion with different concentrations of rotundifolone (3x10^-4, n = 9; 10⁻³, n = 5; 3x10^-3 M, n = 11). (C) Representative recording of Ca²⁺ oscillations induced by rotundifolone (3x10^-3 M) in myocytes placed in Ca²⁺-free medium fortified with 2 mM ethylene glycol tetraacetic acid (EGTA). (D) Summary data demonstrating the decreased amplitude of the Ca²⁺ signal induced by rotundifolone in Ca²⁺-free medium (n = 13). The Ca²⁺ oscillations were measured and represented as increases in fluorescence intensity relative to baseline [DF (%) = (F-F0/F0)*100]. The data were examined using unpaired Student's t tests (rotundifolone with or without EGTA) and one-way ANOVA followed by the Bonferroni post-test. (** p<0.01 and ***p < 0.001 vs control; ###p<0.001 vs Rotundifolone 3x10^-3 M).