Skip to main content
. 2015 Nov 24;10(11):e0143526. doi: 10.1371/journal.pone.0143526

Fig 2. Time course responses of MUC5AC induction to TGF-β and tunicamycin (A and C), and inhibition of MUC5AC induction by kaempferol (B and D).

Fig 2

BEAS-2B cells were treated with 10 ng/ml TGF-β or 1 μM tunicamycin up to 72 h in the absence and presence of 1–20 μM kaempferol. For the measurement of MUC5AC induction, total cell lysates were subject to Western blot analysis with a primary antibody against MUC5AC. β-Actin was used as an internal control. The bar graphs (mean ± SEM) in the bottom panels represent quantitative results of blots. Values not sharing a common letter are significantly different at P<0.05.