Table 1. Influence of the haematocrit on ifosfamide blood determination.
| Sample haematocrit (%) | |||||||
|---|---|---|---|---|---|---|---|
| A. 30% HTC | 20 | 25 | 30 | 35 | 40 | 45 | 50 |
| Mean ± SD (ng/mL) | 4528.9 ± 54.0 | 4830.1 ± 38.3 | 4993.2 ± 18.3 | 5162.6 ± 121.8 | 6249.2 ± 266.8 | 6446.7 ± 340.3 | 6755.0 ± 460.4 |
| % Deviation | -9.4 | -3.39 | -0.14 | 3.25 | 24.98 | 28.93 | 35.1 |
| A. 45% HTC | |||||||
| Mean ± SD (ng/mL) | 3690.0 ± 156.4 | 3861.6 ± 139.5 | 4315.2 ± 151.7 | 4829.8 ± 77.7 | 4899.3 ± 59.4 | 4993.0 ± 69.1 | 5119.8 ± 116.5 |
| % Deviation | -26.20 | -22.76 | -13.69 | -3.40 | -2.01 | -0.13 | 2.39 |
SD: Standard Deviation, % Deviation after triplicate quantification of a nominal concentration (5000 ng/mL) with a calibration curve constructed with 30% (A) and 45% (B) haematocrit, it was calculated as: (theoretical concentration minus calculated concentration/theoretical concentration)*100 [6].