Fig 3. Maximum binding of differently loaded CD1d dimers.

(A+B) Highest relative staining reached with each CD1d dimer loaded with indicated glycolipid as presented in Fig 2. Presented (A) as absolute or (B) as relative to the highest value reached for each CD1d. Human CD1d: Binding maxima are for all glycolipids are reached with Tyloxapol as surfactant. Mouse CD1d: Binding maxima with αGC and PBS57 are reached with Tween 20, forDB01-1 with Triton X-100 and for PBS44 with Tyloxapol. Rat CD1d: Binding maxima for αGC, DB01-1 and PBS44 are reached with Triton X-100, and for PBS57 with Tyloxapol. Cotton Rat: All maxima reached using Tyloxapol. Statistical analysis can be found in S2 Table. Hierarchy of surfactant efficacy between experiments are shown in S1 Fig. Data were computed from results shown in Fig 2.