Table 3. Validation of known and novel miRNAs by Stem-Loop qRT-PCR.
| Novel miRNAs in LV | ||
| miRNA | Ct | Std Dev |
| miR-146b-5p | 33.129 | 0.398256617 |
| miR-151-5p | 26.257 | 0.634608124 |
| miR-378-3p | 20.907 | 0.181923092 |
| miR-423-5p | 29.107 | 0.114140675 |
| miR-145-5p | 27.725 | 0.284937142 |
| miR-222-3p | 30.578 | 0.713898269 |
| miR-330-3p | 30.898 | 0.977237439 |
| miR-324-5p | 26.172 | 1.767929513 |
| miR-2285x-3p | 28.181 | 0.25621647 |
| miR-4005-5p | 28.181 | 0.25621647 |
| U6 snRNA (control) | 25.338 | 0.144719684 |
| Known miRNAs in LV | ||
| miRNA | Ct | Std Dev |
| miR-21-5p | 26.25 | 0.221885857 |
| miR-493-5p | 34.25 | 0.41585374 |
| miR-493-4p | 21.73 | 1.291861135 |
| miR-133-3p | 14.44 | 2.278697632 |
| U6 snRNA (control) | 18.59 | 1.383107503 |
The experiments confirmed the expression of 10 novel and 4 known miRNAs in the left ventricle (LV). For each miRNA and the control U6, the table reports the threshold cycle (Ct) and the standard deviation (Std Dev).