Table 2. Congruence of key diagnostic characters for morphology, biogeography and genetic distances between species resolved by the phylogeny (12S + 16S + COI).
| Character | P. limoncochensis (Clade A) | P. omeviridis (Clade B) | P. acuminatus (Clade C) | P. enigmaticus (Clade D) |
|---|---|---|---|---|
| Skin texture | Smooth | Smooth | Shagreen | Shagreen |
| Tympanic annulus beneath skin | Absent | Present | Absent | Present |
| Males with vocal slits and nuptial pads | Absent | Absent | Present | Absent |
| Tarsal fold or tubercles | Small tubercles | Large tubercles | Smooth | Small tarsal fold |
| Distribution in Amazonia | Northern Ecuador and southern Colombia | Northern Ecuador and southern Colombia | Central and southern Ecuador; northern Peru | Central and southern Ecuador; northern Peru |
| Elevational range (average±sd) | 199–593 m (275.8±75.6) | 154–382 m (251.4±52.8) | 175–1123 m (413.2±269.2) | 169–956 m (501.3±287) |
| Latitudinal range | N1.1442°–S0.91082° | S0.55689°–S2.14833° | S1.21°–S4.04308° | S1.19972°–S3.34207° |
| 12S p-distance (%) | 1.8–3.3% (2.6±1.1) | 1.8–4.7% (3.3±2.1) | 3.3–4.7% (4.0±1.0) | No data |
| 16S p-distance (%) | 1.6–3.0% (2.46±0.8) | 1.6–3.8%(2.96±1.2) | 2.1–3.8%(2.96±0.9) | 2.1–3.5%(2.80±0.7) |
| COI p-distance (%) | 4.7–8.7% (7.3±2.2) | 4.7–7.9% (6.7±1.8) | 6.3–8.7% (7.5±1.2) | 6.3–8.5% (7.6±1.1) |
The number of base substitutions per site from averaging over all sequence pairs between species is shown as an uncorrected p-distance value (%) by gene.