Fig. 9.

Determining whether all four pentanucleotides in the Site II region of the core origin are required for JCV replication. A. Sequences of the JCV core origin, including the four pentanucleotides in the Site II regions. The transition mutations that are substituted for the individual pentanucleotides (P1-P4) are shaded in gray. B. Results of JCV replication assays conducted with the pFL-JCVori derivatives containing transition mutations of the individual pentanucleotides. As a negative control, a reaction was conducted with a plasmid lacking an origin (i.e., pFL; far left column). As a positive control, an additional reaction was conducted with plasmid pFL-JCVori (second column from the left). The levels of replication obtained with the plasmids containing the transition mutations in the individual pentanucleotides are shown in the following four columns.