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. 2015 Nov 24;10(11):e0143635. doi: 10.1371/journal.pone.0143635

Fig 1. (A) The R+G+ model for evaluation of MM activity.

Fig 1

A mouse is restrained (R+) within a cylinder (internal diameter 2.5 cm), of which the front end is blocked with a thin plastic strip (width 1.5 cm, thickness 1.0 mm, length 10 cm). A mouse that can reach the strip gnaws on it (G+) to escape. Taping the tail of a mouse to the cylinder denies the mouse access to the strip. We termed the former condition R+G+ and the latter R+G-, while R-G- means that mice are put in a cage without restraint. The reduction in the weight the strip reflects the MM activity for the 1 h period the mouse is in place, and the decline in that parameter over time is used to calculate a fatigue index for MMs.