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. 2015 Nov 24;10(11):e0143635. doi: 10.1371/journal.pone.0143635

Fig 3. IL-1 immunolocalization in MMs with or without 2 h of R+G+ activity.

Fig 3

(A) Hematoxylin-eosin staining for MM (R+G-); (B and C) immunostaining of IL-1α and β for MMs of mice in the R+G- group; (D and E) immunostaining of IL-1α and β for MMs of the mice in the R+G+ group; (F and G) enlarged views of rectangles indicated in (D) and (E), respectively; (H to O) confocal image showing immunoreactivity for IL-1 protein (green, H to K: IL-1α and L to O: IL-1β) and Ly6G/C (red) separately (H, I, L, and M) and as a merged image (J and N), respectively. Examples of immunopositive cells are indicated by arrows (IL-1α in F, H, and J) or by arrowheads (IL-1β in G, L, and N). n: nerve and asterisk: blood vessel; Bars = 100 μm for (A to E), and 50 μm for (F to N).