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. 2015 Nov 24;10(11):e0143635. doi: 10.1371/journal.pone.0143635

Fig 6. IL-1 stimulation induced IL-6 production.

Fig 6

(A) C2C12 myotubes secrete IL-6 protein upon IL-1 administration. C2C12 myotubes were treated with various concentrations (pg/ml) of rm IL-1α and β for 2 h. IL-6 protein in the medium was subsequently measured via ELISA. Three wells of a 24-well plate were analyzed for each experimental treatment, and 2 separate experiments were performed. Experimental values are given as the mean ± SE (n = 6). a P <0.001 compared to all other groups, b P <0.001 compared to saline. (B) IL-1β injection induced IL-6 production in skeletal muscles in vivo. WT mice were intravenously injected with rm IL-1β at a dose of 5 ng/g BW, and IL-6 concentration was assayed at 0 and 1 h post-injection. In this experiment, QFM were also analyzed, confirming similar effects of IL-1β on skeletal muscles other than MMs. Experimental values are given as the mean ± SE (n = 5–6). *P < 0.05.