Fig 2. Transcriptional activities analysis of deletion constructs of PPARD promoter carrying the four haplotypes.

(A) Transcriptional activities of a series of deletion fragments determined by luciferase assay in C2C12 myoblasts. Left panel, schematic representation of the deleted fragments linked with the luciferase gene in the pGL3 vector. The nucleotides were numbered relative to the transcription start site that was assigned as + 1. Right panel, the relative activities of a series of deletion fragments of pGL3-1880GT vector determined by luciferase assay. (B)(C)(D) The relative activities of a series of deletion fragments of PPARD promoter carrying the different haplotypes in C2C12, 3T3-L1, and PK cells. Error bar represents mean ± S.D. (three independent replicates per group). Asterisk (*) and (**) represent the significance level at P < 0.05 and P < 0.01, respectively (The same below).