Fig 3. Nuclear extracts differentially bind to the promoter fragments carrying the four haplotypes.

(A) (B) (C) EMSA results showing the binding capacities of four haplotypes to the nuclear extracts in C2C12 myoblasts, 3T3-L1 cells, and porcine LD muscle. (D) The EMSA results showing the binding capacities of nuclear extracts to the promoter fragments with haplotypes AC and GC, Lane 1 and Lane 8 were negative control; Lane 2–4 and 5–7 in turn were sample reactions, mutation competitive reactions, and cold competitive reactions for AC and GC reaction groups, respectively. The probes were incubated with nuclear extracts in the absence or presence of 1-fold excess of various competitor probes (mutant or non-labeled probe). The specific DNA-protein complex bands were indicated by arrows. The sequences of various probes were shown under the panel.