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. 2015 Nov 24;10(11):e0143734. doi: 10.1371/journal.pone.0143734

Fig 4. The SNPs in the 5’ regulatory region of PPARD gene affected transcriptional activation of TCF7L2.

Fig 4

(A) The relative mRNA expression profiles of the porcine PPARD and TCF7L2 gene in nine different tissues. (B) Transcriptional activities of four haplotypes determined by luciferase assay before and after TCF7L2 overexpression in C2C12 myoblasts and 3T3-L1 cells. (C) The protein expression levels of TCF7L2 and PPARD before and after TCF7L2 overexpression in C2C12 myoblasts. C2C12 myoblasts were transfected with pcDNA3.1-TCF7L2 vector and empty vector, and then the total protein extracted after 48 h transfection. The protein levels of TCF7L2 and PPARD genes were analyzed by western blotting. (D) Relative PPARD protein expression levels represented by ratio of detected protein to β-actin protein expression level after TCF7L2 overexpression.