Fig 5. DNA pull-down results showing the interaction of TCF7L2 with the four haplotypes in 3T3-L1 cell in vivo.

3T3-L1 cells were seeded in 10-cm dishes for 48 h, harvested and pull-downed by antibodies. Lane GT, AT, GC, and AC: immunoprecipitation results with anti-TCF7L2 monoclonal antibody. Lane total protein: the analysis of total cell lysates before immunoprecipitation to verify expression of TCF7L2. The sequences of four probes were shown under the panel.