Skip to main content
. 2015 Nov 23;10(11):e0142943. doi: 10.1371/journal.pone.0142943

Fig 4. NatB affects Trpm assembly but is not linked to actin fiber formation.

Fig 4

A. Distribution of actin and Trpm in Mdm20- and Nat5-KD HeLa cells. After fixing HeLa cells with paraformaldehyde, the cells were immunostained with phalloidin (green: a, e, and i) and anti-Trpm (red: b, f, and j) antibodies. Merged images of cells counterstained with DAPI (blue) are shown in panels c, g, and k. Low magnification (b, f, and j) and partial zoom up magnification views (d, h, and l) of the indicated regions (dotted line squares) are shown. Arrows indicate the vacuoles. (Scale bar: 20 μm.) B. Comparison of the number of vacuoles in Mdm20- and Nat5-KD cells. Cells were classified into three categories based on the number of vacuole-like structures formed, category 1 (0 to 2 (white area)), category 2 (3 to 5 (light gray area)) and category 3 (over 5 (dark gray area)). The relative ratios of the three categories were calculated based on total cell number. The data represent the mean ± the S.D. (n = 5). C. Western blots for Trpm and cofilin in HeLa cells transfected with siRNA oligonucleotides.