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. 2015 Nov 23;10(11):e0142943. doi: 10.1371/journal.pone.0142943

Fig 6. Reduction in Rictor expression in Mdm20-KD cells is related to post-translational but not transcriptional regulation.

Fig 6

A. Western blots showing the Rictor and Nat5 protein in Mdm20-, Nat5- and Rictor-KD cells treated with MG132 (0.2 μM) or NH4Cl (7.5 mM). B. Quantitative real-time PCR for Nat5 and Rictor mRNA expression was performed using reverse transcription products obtained from control, Mdm20-, Nat5-, and Rictor-KD cells. GAPDH mRNA was used as an internal control for this assay. The data represent the mean ± the S.D. (n = 3). *P<0.01 and **P<0.0005.