Fig 5. HLA-DRB1*01:01 peptide binding affinity, predicted binding affinity for other HLA-DR alleles, and relation to observed T cell responses.

A. Measured HLA-DRB1*01:01 binding affinities grouped according to the frequency of positive responses, for peptides positive in 3 or more donors (n = 14), peptides positive in 1–2 donors (n = 4) or peptides with no positive responses (n = 7). B. Correlation between measured HLA-DRB1*01:01 binding affinity (IC₅₀) and magnitude of T cell response (IFN-γ SFU/10⁶ cells); Pearson’s correlation coefficient: -0.42 (p = 0.038). C. Correlation between predicted binding affinities for HLA-DR alleles from donors examined in this study and predicted binding affinity for HLA-DRB1*01:01. D. Receiver-operator characteristic (ROC) analysis relating predicted peptide-binding affinities for all peptides tested in each donor with CD4 T cell responses observed in that donor. Top left, ROC analysis for fourteen peptides exhibiting strong responses in multiple donors.