Table 3. Summary of DRB1*01:01 binding predictions and IFN-γ responses.
| Protein Name | ORF | Len 1 | All predicted 2 | Selected 3 | Coverage 4 | Pools 5 | In 1+ donor 6 | In 4+ donors 7 | Peptides 8 | In 1+ donor 9 | In 3+ donors 10 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Glycoprotein Q | U100 | 503 | 608 | 28 | 41 | 3 | 2 | 0 | - | - | - |
| Major antigenic phosphoprotein pp100 | U11 | 647 | 850 | 36 | 44 | 4 | 4 | 3 | 27 | 21 | 2 |
| Phosphoprotein pp85 | U14 | 395 | 602 | 22 | 50 | 3 | 3 | 2 | 15 | 11 | 1 |
| Glycoprotein B | U39 | 830 | 822 | 48 | 45 | 5 | 5 | 1 | 10 | 5 | 1 |
| Glycoprotein H | U48 | 809 | 686 | 45 | 63 | 5 | 5 | 4 | 35 | 25 | 1 |
| Virion transactivator | U54 | 413 | 451 | 23 | 65 | 3 | 3 | 2 | 15 | 13 | 2 |
| Major capsid protein | U57 | 1345 | 1337 | 62 | 44 | 6 | 6 | 3 | 32 | 27 | 6 |
| Large tegument protein | U31 | 2077 | 2069 | 103 | 52 | 10 | 6 | 1 | 9 | 5 | 1 |
| Major DNA binding protein | U41 | 1132 | 1124 | 64 | 58 | 8 | 5 | 0 | - | - | - |
| Immediate early protein1 | U90 | 1078 | 1070 | 32 | 34 | 6 | 3 | 0 | - | - | - |
| TOTAL | 9619 | 463 | 43 | 53 | 42 | 16 | 143 | 107 | 14 |
1. Protein length (aa)
2. All 9-mers used for predictions
3. Number of selected 9-mers (top 5% of predicted 9-mers); these were synthesized as peptides after extension at the N and C-terminus
4. Protein coverage (%) considering all synthesized peptides
5. Total number of pools per protein. All these pools were tested for T cell responses
6. Number of positive pools in at least one donor
7. Number of positive pools in at least 4 donors
8. Total number of peptides in positive pools (4+ donors); these peptides were tested for T cell responses
9. Number of positive peptides in at least one donor
10. Number of positive peptides in at least 3 donors