Fig 4. ¹H,¹⁵N-HSQC of pEAβ(3–40) and Aβ(1–40).

25 μM of the monomerized peptides were dissolved in aqueous solution (50 mM potassium phosphate, pH 2.8) containing 20% TFE. Spectra were recorded on a 600 MHz Bruker spectrometer at 5°C. Overlay of the spectrum of pEAβ(3–40) (red) and Aβ(1–40) (blue) indicate that the pyroglutamate modification affects the N-terminal signals significantly towards E11 as well as V40.