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. 2015 Nov 23;10(11):e0143423. doi: 10.1371/journal.pone.0143423

Fig 4. Residues V267 and L276 are essential for SHARPIN-mediated integrin inhibition.

Fig 4

(A) FACS analysis of CHO cells overexpressing GFP alone, or WT or mutant GFP-SHARPIN, together with RFP-TALIN head. The Integrin Activation Index was calculated by dividing active cell-surface integrin levels (FN7-10 binding minus FN7-10 binding in the presence of EDTA) by total cell-surface integrin levels (Mb1.2 staining minus secondary antibody alone) (n = 3). (B) Quantification of migration speed of cpdm MEFs overexpressing GFP alone or GFP-SHARPINWT on 50 μg/ml collagen (n = 78 and 83 cells, respectively). (C,D) Quantification of migration speed (n = 27–125 cells) (C), and representative cell tracks (D) of cpdm MEFs overexpressing WT or mutant GFP-SHARPIN on 50 μg/ml collagen. All numerical data are mean ± s.e.m. ***: p<0.001, *: p<0.05.