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. 2015 Nov 23;10(11):e0143459. doi: 10.1371/journal.pone.0143459

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© 2015 Guillon et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A) Schematic representation of the PCR genotyping strategy. A multiplex PCR was setup with a combination of 3 different primers (arrows) binding to exon 1, intron 1 or the neo cassette that allowed to screen the loss of the wild-type (WT) allele. B) Agarose gel showing genotyping by PCR of CFTR ^+/+ (single band at 1.2 kb), CFTR ^+/- (double band at 1.2 and 0.58 kb) and CFTR ^-/- (single band at 0.58kb) piglets.