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. 2015 Nov 24;10(11):e0143623. doi: 10.1371/journal.pone.0143623

Fig 6. qPCR analysis of the expression levels of m-KL and s-KL transcripts in animals exposed to continuous moderate exercise.

Fig 6

(A) Young C57BL/6 mice started daily exercise at 6 months of age, and (B) old C57BL/6 mice started daily exercise at 12 months of age. Moderate exercise was performed daily throughout the experiments (6 months) using a running wheel. Animals of the young group were sacrificed at 12 months of age, and animals of the old group were sacrificed at 18 months of age. s-KL and m-KL transcripts from the prefrontal cortex, cortex, hippocampus, and cerebellum were quantified by qPCR. Results are compared with siblings not exposed to moderate exercise. m36B4 was used as a reference gene. (C) Immunoblot analysis of s-KL from the prefrontal cortex and hippocampus of sedentary and exercised mice using the K113 antibody. Actin was used to normalize the amount of protein analyzed. N = 6 to 8; *p<0.05 **p<0.01 **p<0.001 by one-way ANOVA with Tukey post-hoc analysis for qPCR, and Student's t-test for immunoblotting. Error bars indicate SEM.