Figure 3. Expression of either FAM150A or FAM150B is sufficient for the activation of wild-type ALK.
(A) Ectopic expression of either FAM150A or FAM150B together with the wild-type human anaplastic lymphoma kinase (ALK) receptor in the Drosophila eye with the GMR-Gal4 driver disrupts the highly organized pattern of ommatidia of the Drosophila eye and generates a rough eye phenotype. Images of adult Drosophila eyes ectopically expressing wild-type ALK in the presence of either FAM150A or FAM150B are shown. Controls expressing either wild-type ALK, FAM150A or FAM150B alone do not display a rough eye phenotype. The constitutively active ALK-F1174S mutant was employed as positive control. (B) Phenotypes observed in adult flies upon GMR-Gal4 driven expression of either FAM150A or FAM150B together with the wild-type ALK at two different temperatures, 18°C and 25°C.