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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Calcium. 2015 Sep 25;58(6):598–605. doi: 10.1016/j.ceca.2015.09.003

Fig. 1.

Fig. 1

Characterization of spontaneous and TRH-stimulated electrical activity in TαT1 cells. (A) The electrical activity measured in current clamp mode in nystatin perforated patch showed a resting membrane potential of −55 ± 4 mV and spontaneous activity, characterized by small (< 10 mV; mean frequency 0.3 Hz) and big depolarizing events (> 10 mV; frequency 0.08 ± 0.03 Hz). (B) The TRH stimulation (100 nM) induced a depolarization of 10 mV and increased the firing frequency of the action potentials to 0.4 ± 0.1 Hz, with amplitudes of 35 ± 6 mV. (Inset) Frequency of spiking in unstimulated and TRH-stimulated cells.