Fig. 8.

Effects of triiodothyronine (T₃) on TRH induced [Ca²⁺]_i response in TαT1 cells. Prior to measurements, the cells were cultured for 48 h in medium containing fetal bovine serum treated with charcoal. Shown are average values of three control experiments (black traces) and three experiments where the cells where pre-treated for 30 min with 10 nM T₃ (gray traces). Average values are derived from at least 20 cells. The arrow indicates the time of application of 10 nM TRH. The dye used is Fluo-3 and [Ca²⁺]_i profiles are presented as ratios between fluorescence intensities and basal fluorescence at the corresponding region. Further background subtraction was also done.