Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Clin Immunol. 2015 Sep 25;161(2):291–299. doi: 10.1016/j.clim.2015.09.008

Figure 2. Misoprostol significantly increases LPS-inducible intracellular cAMP and nuclear pCREB levels.

Figure 2

(A) RAW 264.7 cells were treated with LPS with and without Misoprostol pretreatment for 3 hours and intracellular cAMP levels were measured by cAMP kit. Data are presented as mean±SD, n=3. P<0.01 compared to LPS alone. (B) Human PBMCs were treated with 1 ug/ml LPS for 30 minutes with and without Misoprostol pretreatment and nuclear pCREB levels were analyzed by Western blot. (C) Murine macrophages (RAW 264.7) were treated with PKA inhibitor H89 before Misoprostol (10 µM) and further stimulated with LPS (100ng/ml) for 30 minutes. Nuclear pCREB levels were analyzed by Western blot.