Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Clin Immunol. 2015 Sep 25;161(2):291–299. doi: 10.1016/j.clim.2015.09.008

Figure 3.

Figure 3

Misoprostol significantly attenuates LPS-inducible TNF expression in a PKA dependent manner. (A) PBMCs were pretreated with misoprostol and stimulated with LPS, 1 µg/ml. TNF mRNA expression was examined by real time RT PCR after 3h of LPS stimulation. (B) Cell-free supernatant was collected 6 hours after LPS stimulation, and TNF protein was measured using ELISA kit.***P<0.001 compared to untreated (UT), aP<0.001 compared to LPS. Data are presented as mean±SD, n=3. (C) TNF mRNA levels were analyzed by real time PCR in RAW 264.7 cells treated with LPS for 2 hours with and without Misoprostol pretreatment. ***P<0.001 compared to untreated (UT), aP<0.001 compared to LPS. D. RAW cells were pretreated with H89 (10 µM) before Misoprostol and further stimulated with LPS (100 ng/ml) for 6 hours. TNF production was measured in cell free supernatants using TNF ELISA kit. Data are presented as mean±SD, n=3. ***P<0.0001 compared to LPS, aP<0.001 compared to LPS, bP<0.001 compared to Mis+LPS.