Figure 4. Misoprostol does not affect IκB degradation and p65 nuclear translocation in response to LPS.
(A) RAW cells with and without Misoprostol pretreatment were stimulated with LPS (100 ng/ml) for indicated times, and cytoplasmic IκB levels were analyzed by Western blot. (B) Nuclear p65 levels in RAW cells after 30 minutes of LPS treatment with and without Misoprostol pretreatment. Histone 3 was used as a loading control.