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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Oct 18;1863(1):1–9. doi: 10.1016/j.bbamcr.2015.10.005

Table 3.

Primers used for promoter cloning and mutagenesis analysis. Mutated Sp4 binding sites are underlined with mutated nucleotides in bold.

Gene Promoter Primer
Gabra1 F: 5’ CAGACGCGTTGCTTCCTAGCTTGCGTTCA 3’
R: 5’ CAGCTCGAGGTGCTCCTGCACTGGAGATT 3’
Mutant Sp Gabra1 F: 5’ GCGCTGCATATAAAAAATGTGATGATTGGTGTCCAGATCCAGTGGCTG 3’
R: 5’ CAGCCACTGGATCTGGACACCAATCATCACATTTTTTATATGCAGCGC 3’
Gabra2 F: 5’ CAGGGTACCAAGGCGTGATTAGCCCCTTC 3’
R: 5’ CAGAAGCTTAAGACCAGGCAGCAAACGAA 3’
Mutant of Gabra2 F: 5’ GGCTTGGCCAGGATTGGGTTTTGGGATAAGGGGTCCCC 3’
R: 5’ GGGGACCCCTTATCCCAAAACCCAATCCTGGCCAAGCC 3’