Figure 5.

Hpb Infection Fails to Attenuate Allergic Airway Inflammation in Ffar3^−/− Mice

C57BL/6 wild-type or Ffar3^−/− SPF mice were infected or not with Hpb, then subjected to HDM sensitization and challenge. Mice were sacrificed 3 days after the final HDM challenge and the severity of airway inflammation determined.

(A) Differential cell counts in the BALF. Abbreviations are as follows: Mac, macrophages; neut, neutrophils; eos, eosinophils; lymph, lymphocytes.

(B) Percent eosinophils within BALF cells.

(C and D) Concentrations of (C) IL-4 and (D) IL-5 cytokines within the BALF.

(E) Measurement of HDM-specific IgG1 in the serum. Abbreviation is as follows: OD, optical density.

(F) Mean gross lung histological pathology scores with representative pictures of H&E-stained lung tissue. Scale bars represent 1 mm.

(G) Mean goblet cell index with representative pictures of periodic acid-Schiff-stained lung tissue. Scale bars represent 200 μm. Data are expressed mean ± SD (n = 5).

Each symbol represents an individual mouse. Statistical significance was determined with two-way analysis of variance (ANOVA) or Student’s t test (E), ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Data are from one experiment and are representative of three (A–E) or two (F and G) independent experiments. See also Figure S4.