Figure 1.

Oxtr expressed neurons in the rostral raphe pallidus (rRPa) were activated by cold exposure. (A,B) Cryosections of the RPa, indicating which of the Oxtr-Venus mice were immunostained with anti-GFP. Oxtr was mostly expressed in the rRPa (A), but not in the caudal raphe pallidus (cRPa) (B). Distance from bregma (millimeter) is indicated in the figure panel. (C–F) Oxtr-expressing neurons were activated during the cold condition, but not under normal conditions in the rRPa and RMG. Samples were immunostained with anti-GFP (green), anti-c-Fos (magenta), and merged. The arrows indicate neurons expressing both Venus and c-Fos. (G–I) Oxtr-expressing neurons were immunostained using both glutamatergic markers (vGluT3) and serotonergic markers (TPH2). Scale bar: 100 μm (A–G), 50 μm (H,I).